The regional master teacher studio is a growth community that nurtures regional teachers. It has the characteristics of being interdisciplinary and leading, which is conducive to building a high-quality and professional teaching staff. As the vanguard of educational innovation, it shoulders the mission of exploring new teaching models and promoting high-quality development of education. It should be rooted in local characteristics, break down disciplinary barriers, and take innovative collaboration mechanisms as the key point to carry out in-depth practical exploration of interdisciplinary integration. Based on this, this paper conducts research on the cross-disciplinary integration and innovation practice of the regional master teacher studio, systematically sorts out the practice path, explores the deep value and development path behind it, and provides useful references for educational reform in the new era.