To address issues such as the disconnect between traditional environmental health experiment courses and public health practice needs, insufficient interdisciplinary integration, and a lack of international perspective cultivation, this study focuses on four core objectives and carries out systematic reform practices in teaching models and methods. By constructing a four-in-one practical teaching system integrating “curriculum experiments, scientific research training, social practice, and international collaboration,” developing an interdisciplinary practical case library that incorporates real public health issues, and creating a collaborative innovation and entrepreneurship training model for “undergraduates, postgraduates, and international students,” we aim to achieve a transformation in experimental teaching from knowledge transmission to ability cultivation and from a single mode to a diversified and collaborative approach. Innovations in teaching models and methods are designed to enhance students’ practical skills in public health, scientific research innovation abilities, and cross-cultural collaboration skills, providing replicable and scalable teaching solutions for cultivating top-tier talents in the field of public health.