Objective: To analyze the application effect of comprehensive and refined management in the quality management of unpaid blood donation at blood stations. Methods: A total of 330 unpaid blood donors at our blood station from July 2024 to July 2025 were selected and randomly divided into two groups, with 165 donors in each group. The control group received conventional management, while the observation group received comprehensive and refined management in addition. The success rate of one-time puncture, the occurrence of adverse events after blood collection, the blood discard rate, and the quality of the blood collection and supply environment were compared between the two groups. Results: The success rates of one-time puncture in the observation group and the control group were 98.19% and 93.33%, respectively, and the incidence rates of adverse events after blood collection were 5.45% and 18.79%, respectively (P<0.05). In terms of blood discard, the incidence rates of confidential blood discard, non-standard blood, clotted blood, abnormal plasma color, and abnormal testing process in the observation group were 1.21%, which was lower than the 6.67% in the control group (P<0.05). The quality pass rates of the observation group in terms of the air in the blood collection and supply environment, the surface of environmental objects, the skin of donors' arms, the hygienic hands of blood collection nurses, blood storage refrigerators, and blood specimen transport boxes were all higher than those of the control group (P<0.05). Conclusion: Implementing comprehensive and refined management in the management of unpaid blood donation at blood stations can improve the success rate of blood collection operations, reduce adverse events and blood discard, and improve the quality of the blood collection and supply environment, thereby comprehensively enhancing the quality management level of unpaid blood donation.