Quantitation Method Research for the Pyrrolizidine Alkaloids (PAs) Determination in Botanicals by HPLC-MS/MS

A HPLC-MS/MS method for the determination of Pyrrolizidine alkaloids (PAs) in green tea, orange pekoe tea, hibiscus flower, chamomile flower, and cardamom seed was developed and validated. Its precision, accuracy, linearity, specificity, LOQ, LOD, and ruggedness were validated following AOAC Guidance for Single Laboratory Validation Procedure. The recovery of the analytes is in a range of 70%–130%. LOQ for 21 PAs in the above botanicals is in a range of 0.8 to 6.5 ppb.

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