Effects of Concentration of Rhodamine B in CPPO Solution on the Chemiluminescence Intensity

In chemiluminescence reactions (CL), the electron jumps to a higher energy level when the fluorescent dye is excited. Dye in an excited state emits light by electrons jumping back to the ground state. In this review, indirect CL was studied with the main focus on the curve of CL intensity due to different concentrations of Rhodamine B (RhB) in Bis (2,4,5-trichloro-6-CarboPentoxyPhenyl) Oxalate (CPPO) solution with hydrogen peroxide (PO-CL). Because the light produced after the excitation of the dye by CPPO-CL is visible, the experiments were analyzed directly using a camera and programming. It was observed experimentally that the CL intensity would increase and then decrease with an increasing concentration of RhB. The resulting data are partially consistent with a Gaussian fit, but after excitation, the trend is more likely to fit consistent with that of logarithmic functions. As the CL intensity was reduced at an RhB concentration of 0.05g/20ml, there is a possibility that the quenching effect exists in the reaction of a high RhB concentration with CPPO. The difference in emission wavelengths and the time to reach maximum brightness are also discussed in this report.

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