Autosamplers are indispensable key equipment in modern laboratories, playing a pivotal role in fields such as biomedicine, environmental monitoring, food safety, and materials science. However, domestic autosampler enterprises are facing formidable challenges, confronted by the technological barriers and brand dominance of international giants, as well as increasingly fierce homogeneous competition in the domestic market. This article aims to thoroughly analyze the current market landscape and, based on seven key dimensions—strategic positioning, product technology, sales channels, brand building, service and support, supply chain optimization, and talent development—propose a series of effective market-winning strategies. This will provide theoretical guidance and practical reference for domestic autosampler enterprises to achieve breakthroughs and sustainable development amidst fierce market competition.