The Spatial Location of Chromosomes in Dividing Cells and the Relative Stability of Chromosome Spatial Structure

Chunxiao Wu; Dongyun Jiang; Tao Jiang; Luxia Xu; Qian Xu; Meng Zhao; Qin Zhu; Zhigang Guo; Jinlan Pan; Suning Chen

doi:10.26689/par.v9i2.9965

Chunxiao Wu Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu Province, China
Dongyun Jiang Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu Province, China
Tao Jiang The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, Jiangsu Province, China
Luxia Xu Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210000, Jiangsu Province, China
Qian Xu The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Second Chinese Medicine Hospital, Nanjing 210000, Jiangsu Province, China
Meng Zhao Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210000, Jiangsu Province, China
Qin Zhu Experiment Center for Science and Technology, Nanjing University of Chinese Medicine, Nanjing 210000, Jiangsu Province, China
Zhigang Guo Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210000, Jiangsu Province, China
Jinlan Pan Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu Province, China
Suning Chen Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu Province, China

DOI: https://doi.org/10.26689/par.v9i2.9965

Keywords: Chromosome, Human genetics, Chromosome territory, 3D-FISH

Abstract

The stability and evolution of human genetics depend on chromosomes and chromosome-chromosome interactions. We wish to understand the spatial location of chromosomes in dividing cells in order to understand the relationship between chromosome-chromosome interactions and to further investigate the role of chromosomes and their impact on cell biological behavior. In this study, we explored the relative spatial positional relationships of chromosomes [t(9;22) and t(15;17)] in B-ALL cells by using the three-dimensional DNA fluorescent in situ hybridization (3D-FISH) method. The results showed that chromosomes [t(9;22) and t(15;17)] showed relatively stable spatial relationships. The relative stability of the spatial location of chromosomes in dividing cells may be relevant to disease.

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