A Proposed Solution to the Interference of IgG Kappa-Type M Protein on LDL-C Detection

Chao Yang; Yang Yu; Shuxin Liang; Haige Zhao; Na An

doi:10.26689/par.v7i2.4736

Chao Yang Department of Laboratory Medicine, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
Yang Yu Department of Laboratory Medicine, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
Shuxin Liang Department of Laboratory Medicine, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
Haige Zhao Department of Laboratory Medicine, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
Na An Department of Laboratory Medicine, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China

DOI: https://doi.org/10.26689/par.v7i2.4736

Keywords: M protein, Interference, Biochemical detection

Abstract

Objective: To explore the interference of monoclonal immunoglobulin (M protein) on the detection of serum LDL-C in patients with multiple myeloma, improve the understanding of this matter, determine and establish the correct method, and provide more accurate clinical results through this case. Methods: A case was selected for analysis by the direct method. Results: The interference of IgG kappa-type M protein on LDL-C detection could not be completely eliminated by the enzymatic method. Conclusion: IgG-type type M protein affects the detection of LDL-C by the enzymatic method; thus, light reagents can be used with the direct method for detection.

References

Zhang Y, Liu F, Tian H, et al., 2021, Clinical Significance of M Protein Concentration Detection in Patients with Monoclonal Immunoglobulinemia. Journal of Nephrology and Dialysis Kidney Transplantation, 30(2): 130–35.

Zhuang Y, 2020, Observation on the Effect of Hemolysis and Lipidemia in Serum Samples on the Results of Biochemical Tests. Clinical Research, 18(2): 112–113.

He J, Wang Z, Hu Y, 2013, Interference of IgM M Protein on Clinical Biochemical Detection. Chinese Medicine Guide, 11(15): 432–434.

Li J, Wang L, Zhao X, et al., 2011, Interference and Analysis of Rare IgA M Protein to Clinical Chemical Detection. Marker Immunoassay and Clinical Analysis, 18(6): 398–402.

King RI, Florkowski CM, 2010, How Paraproteins Can Affect Laboratory Assays: Spurious Results and Biological Effects. Pathology, 42: 397–401.

Yang Y, Howanitz PJ, Howanitz JH, et al., 2008, Paraproteins Are a Common Cause of Interferences with Automated Chemistry Methods. Archives of Pathology & Laboratory Medicine, 132(2): 217–223.